Quantification of Opiates in Hair Samples

Quantification of Opiates in whisker SamplesThe analysis of controlled medicine misapply has been primarily been carried pop out u violateg urine precedents. This is accordingly(prenominal) complemented pull ahead with eludingful of some otherwisewise biologic fluids such as blood, sweat and saliva. There ar m whatever advantages as to wherefore urine is a better biological fluid to use in comparison to the other biological fluids. nigh these includeLess invasive No expectles required to obtain the test. inadequate medical supervision requiredCost of conducting the test is loweruranalysis however to a fault has some limitations, some which atomic number 18The medicine maculation window (DDW), the time frame in which the drug chamberpot be describeed is somewhat short. It is typic onlyy 1-3 mean solar days.The warning behind be tardily contaminated therefore testing is carried out on an altered prototype.The try out contri besidese be easily changed if it is diluted. 2There argon in like manner gumshoe issues, if improper c argon is interpreted when handling the sample then sin cont influence may lead to infections.Abstinence can as well as contribute inaccurate readings. If prior knowledge of when the test is going to be carried out, the drug user may abstain from victimisation the controlled substance a few day prior to the test being carried out.Consumption of excessive water the user excessive amount of water ar consumed then the sample accustomed may be diluted, therefore providing an inaccurate assiduousness of the drug maltreatment.The lilt off point- Urinalysis t destinations not to hit a low bountiful of a cut off point. This im luck mistake controlled drug abuse with other possible metabolites of food. An example of this is the consumption of poppy seeds. This can be fictitious for morphine abuse.With upgrade developments analytical techniques such as GC/MS cop samples ar now the preferred sa mple to for the analysis of controlled drug abuse. This is then complemented with urinalysis and blood analysis. The advantages using pigcloths-breadth includeDrugs be commonly base in tomentum samples. blur tends to be much of a qualitative test rather than a quantitative. It measures the minginess and frequency of the abuse, not just its presence. 3A hugeer DDW measures the abuse the abuse oer months and years, as appose to days.The chart above shows the concentration of a drug be urine and copper over a period of 12 daysHair is easier to handle poses no threat of infection if genuflect contact is do 4.Hair is a more st commensurate exemplification it has a stable protein organise which cannot be easily contaminated 5 flyspeck medical supervision or surgical intervention is required when obtaining the sample and is therefore seen to be less invasive.1 HAIR1.1Anatomy of HairHair is made up of devil distinct compartments, the chisel and the follicle/root. The son of a bitch is the visible outer part, which comes out of the skin on the scalp. This part is often propagation referred to as the dead part of blur. The reason for this is that the compartment indoors the skin, the follicle has a medulla shaped ending. Within the centre of the bulb there be cells, which atomic number 18 constantly dividing. As forward-looking cells ar produced the older one be pushed up. As they atomic number 18 moving up they die slowly, which then chance variable the hard shaft.Each strand of sensory hair is made up of protein fibres c solelyed ceratins. The chemic composition of keratin includes long chains of amino acids. One discern example of these amino acids is cysteine, which contains sulphur. One key king of sulphur is its ability to form baffles with other sulphur molecules, disulpher bridges. This is type of chemistry is fall in in hair. Adjacent keratin proteins link together to form disulphide bonds. The molecular inter executes a mid these bonds ar quite strong, and therefore it is quite hard to break the bond betwixt them. The disulphide bonds can be broken using an alkali solution, as acidic solutions generally halt no effect.Each strand consists of triplet seams the cuticle, cortex and medulla. The medulla is made up of cells that argon quite epic and hollow. The middle layer is the cortex, which makes up the majority of hair. The cells in this layer ar tightly jam-packed due to cross links between the keratin chains. The characteristic of hair be predominantly determined by this layer. This includes the glossiness of hair. Other characteristic determined by the cortex argon the tractableness and strength of hair and the similarly the texture.The cuticle is the outermost layer and is organize by a single layer of overlapping tightly packed cells, which are transparent in appearance. This layer provides protection for the cortex and the medulla. This layer also characterises the strength of hai r, as its strenuousness it is able to take the cause of whatever impact.Within the root is the follicle, this is a multilayered bulb like social organisation. Where to each one layer has its own function. At the base of the bulb is the dermal papilla. This is feed by small blood vessels. The function of these vessels is to provide essential nutrients and type O to the increase hair, it also take ups any waste products. This is also the site of where signals are received, instructing hair to grow. 7 This is done by the presence of hormones and adrenogens. The adrenogens determine when hair grows and also the size of the follicle. Therefore influencing the physical properties of hairs, i.e. thickness.The hair follicle is covered by two sheaths, the home(a) and outer. The function of these sheath is to provide protection to the hair shaft. The inner sheath coats the follicle up to the opening of the sebaceous gland. The outer sheath coats the follicle all the elan up to the g land.1.2The Hair CycleEach strand of hair grows in a repeated three stage cycle, offset with the Anagen microscope stage continuing to the Catagen phase and concluding with the Telogen phase. 8Anagen phase Hair growth phaseOccurs with 85 % of hairs at any one timeDuration 2-6 yearsActivity Stimulus received at dermal papilla Rapid fosterage ofkeratinocytes at bottom the bulb Upward passment of keratinocytes Formation of hair shaftCatagen Phase Regression phaseDuration 1-2 WeeksActivity Mitosis cycle ends and resorption breathes The old keratinocytescells are then preceded by the new ones Death of the forwardkeratinocytes Hair follicle size reduced by 1/6 debasement ofthe lower part Hair follicle becomes detached Keratinocytescells attached to the follicle and the dermal make it the next phase.Telogen Phase Rest phaseOccurs with 15% of hairsDuration 3 MonthsActivity Dermal papilla is in a resting state Towards the end of the stage,the hair and follicle become detac hed from one another a new inter- aggroup communication made between the lower part of the follicle and the dermalpapilla once the link has been made, the cycle volition startagain Anagen phase Formation of a new hair, if the previoushair is console attached to the follicle then the new one volition push out theold hair 81.4integration of Opiates Into HairThere have been several studies carried out that look into how drugs and their metabolites are combine into hair. These studies have proposed some simple mechanisms as to how this is done. nevertheless an in depth explanation would require further studies to be carried out.As hair has a protein mental synthesis, it is able to trap the metabolites present in the blood into hair whilst the organise is being unite. This is auctioned whilst the hair is attached to the follicle, i.e. whilst the hair is growing. As the hair fibre is being formed, the drug and its metabolites become comprised. This will government issue in the drug and its metabolites to be stabilised deep down the keratin structure.The primary feign proposes a mechanism that a drug and it metabolites may be integrated by passive diffusion. This is where the drug is passively diffuse from the dermal capillaries into the growing hair cells. The point at which this passive diffusion hands is when the hair is follicle continuance is at a length of approximately 1.2 1.5 mm long. This is the length between the hair matrix area and the area of the keratinised area. This suggests that if the hair is 1.2 1.5 mm long then drug exposure of about three days is open for analysis.A more detailed model can also develop how drugs and their metabolites can be integrated into hair. This takes into account dissimilar mechanisms occurring various times of the hair growth cycle, and at a number of different locations. The explore into this multi-compartment theory was initiated by Henderson 10, but has since been backed up by further studies .An example of this is, the movement of the drugs and its metabolites from sweat and Sebum. The integration of the drugs and their metabolites occurs once the hair has been formed. Studies have shown that the concentration of drugs in sweat is higher than the concentration free-base in blood. This would therefore explain the high concentrations if drugs install in hair. 10 Drugs can also be integrated into hair from the outside milieu, i.e. from air, water and hair treatments such as hair dying, and perming.As rise up as the external environment drugs can also be integrated with hair by intradermic transfer. This happens deep inwardly the skin compartment, where highly lipid soluble drugs can penetrate into the skin layer and then become integrated into hair. 11 excessively melanin content may have some influence on the drug being transferred. The drug may associate with melanin sites that are present in the skin. This will result in the transfer of the drug and its metaboli tes as well as melanin pigment molecule.The actual properties of the drug being integrated will also ultimately influence which mechanism is used for the drug to be integrated with hair. Examples of these properties include the geomorphologic, chemical, and physical properties. When looking specifically at the structural properties, there are three factors that will influence the mechanism under taken to integrate the hair. 13 These includeThe melanin content of the hairThe lipophilicity of the drugThe basicity of the drugThe influence of melanin on the integration of the drug with has been examined in several studies. A sample of colorise hair was analysed. It can be seen that the sample contains discolor hair and pigmented hair. It was rear that blush though the root had been placed under the identical conditions, i.e. the corresponding concentration of the drug and its metabolites in the blood the pigmented had ten times the concentration of the basic drug compared to the h air sample that was not pigmented. 14 This field carried out by M. Rothe et al prompted further studies to be carried out. These looked at the difference in drug concentration between black, brown, blonde and red coloured hair. The results obtained from this study also found the correlating results.The integration of uncharged organic i.e. lipophilic drugs can infiltrate the membrane with ease, as well as being able to diffuse on the concentration gradient. This however is not the lawsuit with lipophobic or charged drugs. When they try to infiltrate the membrane a drug broad barrier is formed, therefore restricting the drug from ventureing the membrane. in any case basic and acid drugs, are highly ionised can enter the membrane if the charge they have is neutralised. This is achieved by deprotonation or protonation. This suggests that the pkA of the drug is an important factor, when it is nerve-racking to enter the melanocyte cells so it can be integrated with hair.Studies h ave also found that the intracellular pH of melanocytes typically ranges from 3 to about 5. delinquent to this chemical property, there is an increase in the accumulation of drugs at pigmented sites. However this is not the case for all acidic drugs, so this is why they are often found in lower concentrations. 132. Opiates and Opioids2.1Derivation and active functionThe opiates are derived from opium. Opium is released from immature seeds that grow within the poppy plant, also known as papaverus somniferum.The active component from which the opiates are synthesised, is known as the latex. This is a white milk like emulsion fluid that is released, when an incision made on the green wall of the poppy plant seed. The latex is rack upd typically between 1-3 weeks after the poppy plant has flowered.The white latex is then dried, leading to the validation of brown coloured opium. They are a group of about twenty dollar bill opiate alkaloids. An opiates can however is a man-made chemi cal/drug that can be synthesised using an opiate as starting material, or be full synthesised to mimic the action of an opiate. Morphine is the most prominent opiate present within opium, making up 10%. Codeine is second, which makes up approximately 5% of opium. The other main constituents of opium include thebaine, noscapine and papaverine. whatever of the twenty alkaloids can be synthesised further in laboratories They can be synthesised using an opiate as starting material, or be fully synthesised to mimic the action of an opiate.An example of this is the synthesis of diacetylmorphine from morphine. There are also opioids that can be synthesised fully in a laboratory. An example of this type of opioid is methadone.2.2ClassificationThe opiates can be assort into three main categories, born(p) opiates, semi celluloid opioids and fully synthetical opioids.2.2.1Natural OpiatesThese are chemical/drugs that are synthesised directly from the latex that is produced from the seedling s of the poppy plant. Once the latex has been dried it is now known as opium. The natural opiates are then extracted from the dried opium. The most abundant chemical/drug present in the opium is morphine, news report for 10 % of opium. The second most abundant natural opiate is codeine, account for approximately 5 % of opium. Thebaine is the third most abundant opiate, accounting for approximately 3% of opium.ThebaineThe chemical composition of morphine and codeine is quite complex. This is why it is not feasible to synthesise them in a laboratory. This therefore way that the best method of obtaining these opiates is through direct parentage from the poppy plant.2.2.2Semi synthetic substance OpiatesThese types of opiates are synthesised using the natural opiates, such morphine as starting points. There are a vast amount of semi synthetic opiates. One example of a natural opiate being used to synthesise a semi synthetic opiate is the production of heroin from morphine.HEROINThe response of morphine with acetic anhydride results in the formation of diacytylmorphine, also known as heroin. Morphine as well as the other natural opiates are the starting material for many semi synthetic opiates. The table infra shows examples of these semi synthetic opiates.Semi Synthetic OpiateAlso Known AsStarting Natural OpiateChemical StructureHydromorphoneDihydromorphinone and DimorphoneMorphine Hydrogenated ketoneHydrocodoneDihydrocodeinoneCodeine and ThebaineOxycodoneDihydrohydroxycodeinoneThebaine The structure is similar to codeine, but differs in 3 ways1 -hydroxyl group at C-14, codeine has a H2- has a dihydro between C 7,8, codeine has ternary C bond3- carbonyl group present instead of a hydroxyl groupOxymorphone14-HydroxydihydromorphinoneThebaine or Morphine Esterification of the hydroxyl groupsDesomorphineDihydrodesoxymorphineMorphine withdraw 6-hydroxy groupSaturation of the 7,8 C double bondHeroindiacetylmorphineMorphine-Addition of acetyl ester groups at C 3, 6, therefore diacetyl ester of morphineCodethylineEthylmorphineCodeine or Morphinethe OC2H5 group substituted for an aromatic 3-OH2.2.3Fully Synthetic OpiatesThe fully synthetic opioids are completely chemically different to opiates, however the mode of action on the ashes. The fully synthetic opiates are able to mimic the way morphine acts on the soundbox. The first type of fully synthetic opiates that was synthesised was called meperidine. This was then with the production of methadone.Some other examples of fully synthetic opiates are fentanyl, pethidine, tramadol and dextropropoxyphene. The advantages of synthesising these synthetic opiates are that the potency of the chemical/drug can be chop-chop increased, in comparison to that of morphine.2.2.4Endogenous OpiatesThese are natural substances that are produced within the brain. The characteristics of the endogenous opiates are similar to that of the alkaloid opiates that derived from the poppy plant, commonly known as exogen ic opiates. The endogenous opiates interact with opiates receptors in the same way as the exogenous opiates i.e. causing analgesia and euphoria. Examples of these endogenous opiates areEndorphinsEnkephalinsDynorphins2.3 Mode of Action of OpiatesOpiates are chemicals that act on the body in two ways. The first is by lessen or stopping chemical signals, therefore having sedative effects. This will result in a reduction reaction time in which the body reacts to pain, also helps to decrease the awareness of pain and finally helps increase the tolerance of pain. The second way in which the opiates act within the body is to develop a toneing of elation.The mechanisms that allow the opiates to behave this way is achieved by the interactions that occur at the opiate receptors. The opiate receptors are located mainly in the central anxious(p) system, i.e. the brain and spinal cord and also within the respiratory centre. The body also produces it own natural opiates, known as endogenous opiates. Some examples of these endogenous opiates are endorphins, enkephalins and dynorphins. They are all released naturally to interact with the opiate receptors. The endorphins are located in the hypothalamus, and are released in response to stress. The enkephalins are present within the central nervous system, and act on the pain pathways. The dynorphins are also located in central nervous system, the spinal cord. They are also associated with the pain pathways.These natural opiates interact with three main opiate receptors mu, kappa and delta, which are g-protein coupled.The opiates that are derivative of the poppy plant are called exogenous opiates. They also interact with the mu, kappa and opiate receptors. If the use of the exogenous opiate s is abused, adverse effects. As well as the opiates being able to block pain, they also make the user feel elated. This is the result of the opiates reacting with mu opiate receptors. The same receptor that the endogenous opiates, endor phin reacts with. Due to these properties it is often the case that opiates are used recreationally as appose to medically.3. Extraction of Opiates from Hair3.1In order to determine the presence of in a hair sample, the drugs need to be extracted from the hair structure. The reason for this is that there have not been any developments in analytical techniques that analyse the hair and drug when they are combined in one structure. This is why declension steps are taken to analyse the drug separately from the hair structure. The choice of solvent used for the extraction surgical process must take into consideration the chemical structure of the drug, and what response they will have to the solvent.3.2Division of hair in to segmentsHair must be divided into segments prior to the opiates being extracted. As hair grows at a rate 0.5 inches per month ref -see notes, it provides a timeline of when and at what concentration the opiates we consumed. The hair sample must be all be of the sa me length prior to being analysed. It is quite difficult to quantify at which period of time the opiate was consumed if a clump of hair is used as appose to a single strand of hair. it is generally get harder the eight-day the distance from the root. This is why it is beneficial to analyse the hair sample in sections. 25-22Studies carried out, have found the following divisions of a 45 cm length provide the optimum analysis. Staring from the root the following divisions are made4 x 0.5 cm3 x 1.0 cm2 x 2.0 cm2 x 3.0 cm2 x 5.0 cm2 x 10 cm3.3Decontamination of hair anterior to any extraction techniques being carried out on hair, any external contaminants must be removed. Although the analytical techniques analyse the opiates that are incorporated within the hair structure, sometimes other substances may be detected if the decontamination process is not actioned correctly. The results of the analysis may account for surface contaminants that may have made contact with hair, i.e. if the user has touched a substance and by and by touches their hair. This will result in a positive result eng obtained even though the user has not consumed the substance.Other possible sources of these contaminants may be from hair care products such as shampoos and conditioners. Also any hair styling products, such waxes and hair sprays also need to be removed. As well these sweat and any fatty sebum released from the sebaceous glands need to be removed. Also environmental contaminants such dust need to be discarded. If the sample prior to being cut was exposed to any drugs in the environment, this step will remove this source of contamination. The reason for decontaminating the hair sample is to prevent any background noise when the sample is analysed. The choice of the decontaminant has to have specific properties. This is because it has to remove any external contaminants, however not be able to remove any of the drugs and its metabolites from the hair sample. 15Non protic solvent s such as dichlormomethane and acetone are honest decontaminates as they do not fop the hair, so extraction will not occur.Using a 300mg sample of hair is used. It is placed into an ultrasonic bath,There are series backwash cycles performed on the hair sample, and are usually initiated with two washes with dichloromethane. A typical experiment conducted in 16, which move to determine the opiate content in hair carried out four different wash cycle, on four different samples of hair.20 ml of dichloromethane, 15 ml of acetone, 15 ml of wood alcohol, 10 ml of methanol.20 ml of isopropanol, 15 ml of acetone, 15 ml of methanol, 10 ml of methanol.20 ml of dichloromethane, 15 ml of isopropanol, 15 ml of methanol, 10 ml of methanol.20 ml of n-hexane , 15 ml of acetone, 15 ml of methanol, 10 ml of methanol.This experiment showed that a mixture of solvents could be used to wash the hair samples.3.4Disintegration of opiates from hair structureAs there are currently no analytical techniques that test for opiates whilst they are integrated within the hair structure. This means that the hair structure must first of all be digested and then the drugs and its metabolites are extracted, to determine which drugs are present.There are various solvents used to extract opiates and its metabolites from hair.3.4.1Extraction with MethanolMethanol is a good solvent used to extract opiates from hair. It is hydrophilic, so it can enter the hair structure quite easily. The action of methanol is that it causes the hair to swell up. This will result in the drugs integrated within the hair structure to be released. This is done by the opiates diffusing out. This extraction is carried out in an ultrasonic bath. This helps to degrade the hair structure. There are some impurities still present once this methanol extraction has been carried out. So a supplemental clean up is still required. 17There are some disadvantages to this extraction method. This is because the amount of drug obtaine d from the extraction procedure, is quantitatively less than other extraction methods used to derive opiates hair from hair.However the main disadvantage of using is that using methanol extraction, this is that the opiate extracted can sometimes be hydrolysed. An example is the novelty of 6-monoacetylmprphine (Heroin) to morphine. This results in the non detection of monoacetylmprphine (heroin). 13 Therefore when trying to detect the opiate Heroin, it can be hydrolysed to morphine. Therefore resulting in the heroin present in hair to go undetected. 213.4.2Extraction with a buffer solutionThis extraction procedure is wide used to extract opiates and their metabolites from hair. It generally seen to be one the more prospered methods. A typical buffer solution would be a orthophosphate buffer, at a pH of approximately 6.4 7.6. 18 In comparison to methanol phosphate buffer are seen to be a cleaner feeler of extracting opiates. In addition to use the phosphate sometimes additional e nzyme are added to help to determine intricate metabolites. A typical enzymes used are combination of glucuronidase and arylsulphitase.3.4.3Supercritical Fluid ExtractionThis method uses a critical fluids such carbon dioxide (CO2) to extract opiates from hair.It is seen to be advantageous over other extraction methods, as critical fluids have specific properties that allow them to be more efficient in extracting opiates and their metabolites from hair samples. Some examples of these properties include that physically, supercritical fluids are less viscous than other solvents. This in turn allows them to move more freely. 19They have an increased speed of extraction, in particular with opiates. question carried out into the extraction of opiates from hair using supercritical fluids by Edder et al. It was found that use of the supercritical fluid carbon dioxide, not only speeded up the extraction process but also retrieved a high yield. It was found that 100 % of the morphine that was present in the hair sample was extracted, along with 98.2% codeine, and 92% of methadone. This was all done in a 25 arcsecond procedure. 20Other advantages of using supercritical fluids to extract opiates from hair samples are that it has been found that supercritical fluids tend not to contaminate the samples, in comparison to unfluctuating phase extraction and liquid liquid extraction. The efficiency of this method also allows the procedure to be more automated in comparison to other extraction techniques.3.4.4Enzymatic Digestion of the Hair matrixThis method primarily uses the enzymes pronase and protein kinase A to break down the hair structure. The procedure requires the hair sample to be placed into the enzyme mixture at temperature between 40 -60oc for approximately 6 12 hours. 22The action of these enzymes is to breakdown the disulphide bonds that are present within the hair structure. Often dithiothreitol is used to aid pronase and protein kinase A, by decreasing the time taken to extract the opiates and their metabolites from the hair sample.Other enzymes used to breakdown the hair structure include glucuronidase and arylsulphitase.The disadvantage of using this method in comparison to other extraction techniques is that some of the sample may be altered prior to them being for the analytical tests. An example is the antibodies that are required for radio immunoassays, may be denatured by excessive heating required by this extraction process.3.4.5Digestion with Sodium Hydroxide.The use of alkaline solutions such as atomic number 11 hydroxide in digesting hair for the extraction of opiates has proven to be real compatible. This is because unlike acid solvents the constituents of the opiates are not hydrolysed along with the hair structure. An example of study conducted by Aldo Polettini et al found that in some case hair samples of heroin users, when digested in methanol hydrolysed the heroin to morphine. Whereas the hair sample that was dige sted in sodium hydroxide successfully hydrolysed the hair structure but did not alter the opiate and its constituents. refTypical experiments digest hair samples in a 2M concentration of sodium hydroxide, set at a temperature of about 79c for 60minutes.4. Gas Chromatography / Mass Spectrometry Analysis of Hair Samples4.1How GC/MS worksGas Chromatography and Mass Spectrometry are two separate analytical techniques that are used together to quantitatively detect low concentrations of opiates. This analytical technique has exceptional specificity to the detecting in opiates in hair, with levels ranging from nanograms to picograms.4.1.1Gas ChromatographyThe turgidity chromatograph is a heated unit that has thin silicon oxide capillary columns, which have a cross linked silicone layer. The opiate sample is injected into an inlet and heated. The sample is heated until the boiling point of the last part of the opiate sample has been exceeded by approximately 20 C. This is typically betwe en cc 260 C. This leads to the vaporisation of the opiate sample.The vaporised opiate sample will move to the beginning section of the silicon dioxide capillaries. This is aided by an inert gas, typically helium. The temperature is somewhat reduced at the silica capillaries, typically 120C. This will result in the concretion of the opiate sample. The reason for this condensation step is to ensure that all of the constituents of the opiate sample set off forward from a uniform point.The opiate molecule will start to dilapidate as it moves along the capillary column. This disintegration is caused by varied physiochemical interactions that occur with the different constituents of the opiate molecule, during the stationary phase.The time taken for each constituent or metabolite to move of the opiate sample to move through the capillary tube, from the point of shot is referred to as the retention time. 284.1.2Mass SpectrometryOnce the separate constituents of the opiate sample leave the capillary column, they begin to enter the mass spectrometer. The compartment between the gas chromatograph and the mass spectrometer is under high vacuum, which have quadrapoles that cover the end of the silica capillary.Now that the sample is moving along from the GC they are met by a beam of electrons, resulting in the sample to become ionised. The quadrapoles bankrupt the different constituents of the opiate samples, in relation to their electrical charge and their molecular weight. An electrical pulse is generated as the ion sensor acts on the charged opiate constituents. This is all record on to library computer, which generates a spectrum of the opiate constituents behaviour within the mass spectrometer. 29A Typical GC/MS A capillary tube estimator4.2A typical GC/MS procedure on hair samples of opiate abusers.4.2.1Typical GC ConditionsThe type of capillary column used to quantification of the opiates is a HP 5MS, 5% phenyl methyl siloxane, with dimensions of 30m x 0.25 m x 0.25m photo thickness.The temperature of the inlet is set to 230c.The inert gas used was 99.999% helium, which flows at a rate of 1ml/min.The temperature of the oven is held at 150c for 1 minute.The GC is then programmed to increase the temperature in the following increments. 304.2.2Mass spectrometry ConditionsThe mass detector was set up to operate at voltage of 70eV.The temperature at the quadrupol